| Gene | Calml4 |
| Chromosome | 9 |
| Deletion Start | 62,875,590 |
| Deletion End | 62,858,162 |
| Deletion Size | 17,429 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,858,728 | 62,858,748 | CCTCCTGCCTAGTTCAGTGTG |
| TUP | 62,858,751 | 62,858,776 | CACCTTGTTCTTGGGCTTCCCACAGA |
| TUR | 62,858,802 | 62,858,826 | CCTGTGTAACTTAATTCTGCCTTTC |
| TDF | 62,875,047 | 62,875,066 | CCCTGTGACTGGGAGGTTAC |
| TDP | 62,875,067 | 62,875,091 | AAGGGTGGGCTGCACAGGTACAACA |
| TDR | 62,875,140 | 62,875,161 | CCATGTGGGTTCCAGAGATCAG |
PCR Primers
| Primer | Sequence |
| SU | GGAACACCCAAATTGTCCAG |
| SD | CAGGGAAACACCAATTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17781A-A10 | VGB6 | C57BL/6NTac | |
| 17781A-A12 | VGB6 | C57BL/6NTac | |
| 17781A-G4 | VGB6 | C57BL/6NTac | |
| 17781A-E10 | VGB6 | C57BL/6NTac | |
| 17781A-F5 | VGB6 | C57BL/6NTac | |
| 17781A-E11 | VGB6 | C57BL/6NTac | |
