| Gene | 1700016K19Rik |
| Chromosome | 11 |
| Deletion Start | 76,003,485 |
| Deletion End | 76,000,065 |
| Deletion Size | 3,421 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,000,260 | 76,000,279 | GGCGACGGTAAGAGGAACAG |
| TUP | 76,000,286 | 76,000,310 | AGGTTGCTTCGGCTCTTGGACCTGA |
| TUR | 76,000,311 | 76,000,334 | AGGTAGAAGGGTATGGGAGGAATC |
| TDF | 76,003,204 | 76,003,223 | GAGAGTCTGCGTTGGGATGG |
| TDP | 76,003,227 | 76,003,248 | TCTCACCGACCCTGAGGCAGAA |
| TDR | 76,003,273 | 76,003,293 | GTACCGCTTCCTTCGGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTTGTCACTGTCAGCTAAGC |
| SD | AGGAATTCTTGCTGAGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17782A-B5 | VGB6 | C57BL/6NTac | |
| 17782A-B8 | VGB6 | C57BL/6NTac | |
| 17782A-D5 | VGB6 | C57BL/6NTac | |
| 17782A-F2 | VGB6 | C57BL/6NTac | |
| 17782A-E3 | VGB6 | C57BL/6NTac | |
| 17782A-F1 | VGB6 | C57BL/6NTac | |
