17783   3230401D17Rik     Status: ES cell colonies screened / QC positives

Gene3230401D17Rik
Chromosome2
Deletion Start163,415,608
Deletion End163,406,311
Deletion Size9,298
Genome Build38
MGI IDMGI:1913930
NCBI Gene ID66680
BAC ID378I22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,414,811163,414,831AGGCACTGCCCATCGTCTTAG
TUP163,414,782163,414,807AGAGGCTGCTCGCAAAATTAGATCCC
TUR163,414,688163,414,711TGTCTGCAATGGACAACATGAAAG
TDF163,407,290163,407,308CCCATGTCTCCCATGTGTC
TDP163,407,267163,407,289TGGCCTGGGTGAGTATGTCCCCT
TDR163,407,231163,407,250TCAGCATCCCTTGTTAGCAG

PCR Primers

PrimerSequence
SUAACAGCCGTGTATGTGTGTG
SDCCCGTGTTAAACCAAAGACAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR144 bp++-
TDFTDR78 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17783A-A2VGB6C57BL/6NTac
17783A-C12VGB6C57BL/6NTac
17783A-G11VGB6C57BL/6NTac
17783A-F5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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