17785   Arpc5l     Status: ES cell colonies screened / QC positives

GeneArpc5l
Chromosome2
Deletion Start39,015,170
Deletion End39,008,199
Deletion Size6,972
Genome Build38
MGI IDMGI:1921442
NCBI Gene ID74192
BAC ID362H5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF39,008,67739,008,696CCCTACTGCGCTTGTCTAAC
TUP39,008,69739,008,719TGGCTTTCTTGGGCCCGATTGTA
TUR39,008,74339,008,763TCAGTCTTCCTCCAACCATCC
TDF39,014,96639,014,988CTGGGAATATGTGTCCTCATCTC
TDP39,014,99339,015,014TGAGTAAAGCTCCCCGCTGGCC
TDR39,015,06539,015,087AATGGAGCCTAGTCCTCCTACAG

PCR Primers

PrimerSequence
SUTGCCATCCCTTAGCTCTAAG
SDTTTATTGATGCAGCAGGCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR87 bp++-
TDFTDR122 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17785A-H12VGB6C57BL/6NTac
17785A-A8VGB6C57BL/6NTac
17785A-B7VGB6C57BL/6NTac
17785A-C2VGB6C57BL/6NTac
17785A-F11VGB6C57BL/6NTac
17785A-A7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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