| Gene | Bag4 |
| Chromosome | 8 |
| Deletion Start | 25,785,168 |
| Deletion End | 25,767,852 |
| Deletion Size | 17,317 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,784,597 | 25,784,615 | CTGCGCTGACTTCCTAAGC |
| TUP | 25,784,568 | 25,784,591 | AGCAGGCTGCAGGATCTCTTTCCC |
| TUR | 25,784,517 | 25,784,540 | GAGCCACGCAGGATAAATTGTTAC |
| TDF | 25,768,384 | 25,768,405 | CCTCGTACTCCTACAACCCATC |
| TDP | 25,768,360 | 25,768,380 | TCAAGGCCTGAGCCGGCACAG |
| TDR | 25,768,320 | 25,768,340 | TGCTCCCGGAGATTCATACTG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCTCACGCGTGACGAG |
| SD | TGAAGTATCTGCAGTCACAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17788B-F5 | VGB6 | C57BL/6NTac | |
| 17788B-G1 | VGB6 | C57BL/6NTac | |
| 17788B-H10 | VGB6 | C57BL/6NTac | |
