| Gene | Cenpj |
| Chromosome | 14 |
| Deletion Start | 56,565,103 |
| Deletion End | 56,526,910 |
| Deletion Size | 38,194 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,564,806 | 56,564,825 | TCCTCCGGTCTTTCCTTCAG |
| TUP | 56,564,776 | 56,564,803 | AGAAAGGAGCCAATTGCATCACGTCAGG |
| TUR | 56,564,725 | 56,564,747 | CGAGATGAGGCGTAAGATCATTG |
| TDF | 56,527,048 | 56,527,069 | GCTCCATACTGCCCAGTTCAAG |
| TDP | 56,527,016 | 56,527,045 | ACGAGAGTATCCCGATGGGACTGTTAAGAC |
| TDR | 56,526,971 | 56,526,991 | CGGCCAGATGTGTACTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCCAACCAAGCAGTTTAC |
| SD | GAAAACCTGCATAGCAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17791A-C8 | VGB6 | C57BL/6NTac | |
| 17791A-F3 | VGB6 | C57BL/6NTac | |
| 17791A-D6 | VGB6 | C57BL/6NTac | |
| 17791A-C11 | VGB6 | C57BL/6NTac | |
| 17791A-H6 | VGB6 | C57BL/6NTac | |
