| Gene | Crcp |
| Chromosome | 5 |
| Deletion Start | 130,059,896 |
| Deletion End | 130,029,403 |
| Deletion Size | 30,494 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,029,788 | 130,029,808 | GCCAGCATCATCACCATCATC |
| TUP | 130,029,818 | 130,029,844 | TGGTGGAAATGGTACCCAGGACTTTGC |
| TUR | 130,029,855 | 130,029,872 | TGCAGGTCGGTGGCAAAG |
| TDF | 130,059,028 | 130,059,047 | TCCTAGTGTGCCTACAGTCC |
| TDP | 130,059,058 | 130,059,082 | CCCTACAGAGTTCTGGTGAGTGGCA |
| TDR | 130,059,084 | 130,059,106 | ACCTTGCCTTAGTTCTCCTTACC |
PCR Primers
| Primer | Sequence |
| SU | GGGATTTTAATGCGGCCAAG |
| SD | CCTAGAGCAGCTGTGTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17793A-B4 | VGB6 | C57BL/6NTac | |
| 17793A-F8 | VGB6 | C57BL/6NTac | |
| 17793A-H11 | VGB6 | C57BL/6NTac | |
| 17793A-F3 | VGB6 | C57BL/6NTac | |
| 17793A-G3 | VGB6 | C57BL/6NTac | |
| 17793A-G11 | VGB6 | C57BL/6NTac | |
