| Gene | Ermp1 |
| Chromosome | 19 |
| Deletion Start | 29,648,352 |
| Deletion End | 29,612,486 |
| Deletion Size | 35,867 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,647,570 | 29,647,593 | TGGTGTGGGAGAGGAGTAATGTAG |
| TUP | 29,647,542 | 29,647,569 | TGCGTGCTAACCTTTCTAAGCACCACAG |
| TUR | 29,647,515 | 29,647,539 | TCCTTCCACAGCTTTCAGATATGAG |
| TDF | 29,612,777 | 29,612,796 | TGCATGGCGGTTCTGGATAG |
| TDP | 29,612,751 | 29,612,776 | AAGTGCAGGTGGGATTTCCCAGGCTT |
| TDR | 29,612,718 | 29,612,739 | CACTGGCCTCCAAGGTTGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCCAGTATTCATCAGCCC |
| SD | GATCCTCACAGGAAAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17795A-F9 | VGB6 | C57BL/6NTac | |
| 17795A-C1 | VGB6 | C57BL/6NTac | |
| 17795A-H3 | VGB6 | C57BL/6NTac | |
