| Gene | Fyb |
| Chromosome | 15 |
| Deletion Start | 6,619,747 |
| Deletion End | 6,579,951 |
| Deletion Size | 39,797 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,580,654 | 6,580,676 | AACCACCTAAGGAAGACCCAGAG |
| TUP | 6,580,679 | 6,580,706 | TAAAGACCATGGAGCACCAAGTTCACCC |
| TUR | 6,580,722 | 6,580,739 | GCTCGCAGCAGGTTTCAG |
| TDF | 6,619,532 | 6,619,555 | TCAAGTTTGTTTCTGCATCTTTGG |
| TDP | 6,619,570 | 6,619,599 | AATTTACCATCCCTTGCTTCTTAGGTGCCA |
| TDR | 6,619,608 | 6,619,631 | GGAAGTTGTCGAGTAAGGTGTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTCCAACAAGATAGCGAGGC |
| SD | CTGGAAGTAAGAAAGTATGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17799B-H9 | VGB6 | C57BL/6NTac | |
