17800   Gmeb2     Status: ES cell colonies screened / QC positives

GeneGmeb2
Chromosome2
Deletion Start181,278,076
Deletion End181,253,685
Deletion Size24,392
Genome Build38
MGI IDMGI:2652836
NCBI Gene ID229004
BAC ID369K24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFAGTGCTGGGATAAAGAGCATGTG
TUPCCAGGCACTGGCATAGCCTCACA
TURCCCATCCTGATAGAGCTGTCTC
TDFGAGCACACGGCCACTATTGAG
TDPCTGCTGTAGCAGAGGACCATGAGCA
TDRAGGCAGCCAGCCCATCTTG

PCR Primers

PrimerSequence
SUTTCTGGCTAGAAACCCATGC
SDTGTGGGAAGAGACCAAATCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17800A-B2VGB6C57BL/6NTac
17800A-B7VGB6C57BL/6NTac
17800A-C9VGB6C57BL/6NTac
17800A-E10VGB6C57BL/6NTac
17800A-H11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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