| Gene | Mat2a |
| Chromosome | 6 |
| Deletion Start | 72,439,433 |
| Deletion End | 72,434,242 |
| Deletion Size | 5,192 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,438,864 | 72,438,887 | CCGAAGGTCCCATCTCTAATATCC |
| TUP | 72,438,815 | 72,438,844 | AAACGCTTAAGAGCATGGGTAGACATGCCC |
| TUR | 72,438,772 | 72,438,795 | CGCTATATTTAGTCAGTGGCAGTC |
| TDF | 72,434,696 | 72,434,720 | GCTAAAGTGGGATGCTCAAAGTTAC |
| TDP | 72,434,658 | 72,434,686 | AACCATAGTGGTCAGTGATTGGACCTTGG |
| TDR | 72,434,599 | 72,434,619 | CCAGCTCTGGCTTGACAAATG |
PCR Primers
| Primer | Sequence |
| SU | GCGGACCTCCCTGCCAAC |
| SD | TTCAGCATCTGGTGGGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17801A-B9 | VGB6 | C57BL/6NTac | |
| 17801A-D1 | VGB6 | C57BL/6NTac | |
| 17801A-H11 | VGB6 | C57BL/6NTac | |
| 17801A-F5 | VGB6 | C57BL/6NTac | |
| 17801A-G7 | VGB6 | C57BL/6NTac | |
| 17801A-E10 | VGB6 | C57BL/6NTac | |
