| Gene | Myh9 |
| Chromosome | 15 |
| Deletion Start | 77,813,209 |
| Deletion End | 77,783,393 |
| Deletion Size | 29,817 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,812,913 | 77,812,930 | GCTTCGGTGCTGCACAAC |
| TUP | 77,812,883 | 77,812,911 | TCAAGGAGCGATACTACTCAGGGCTTATC |
| TUR | 77,812,831 | 77,812,853 | CACCCAGACAGTGGGATAAAGAG |
| TDF | 77,783,468 | 77,783,488 | GACGAGTGGCTGATGAAGAAC |
| TDP | 77,783,435 | 77,783,460 | CCTTGAACGACAACATCGCCACGCTG |
| TDR | 77,783,395 | 77,783,414 | TCCACAGCTCAGAGACAAAC |
PCR Primers
| Primer | Sequence |
| SU | TTACCAGTGGACTGTGGCTG |
| SD | CTGGGGCTACCTGCGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17803A-B6 | VGB6 | C57BL/6NTac | |
| 17803A-H10 | VGB6 | C57BL/6NTac | |
| 17803A-G8 | VGB6 | C57BL/6NTac | |
