| Gene | Ncbp2 |
| Chromosome | 16 |
| Deletion Start | 31,957,048 |
| Deletion End | 31,948,653 |
| Deletion Size | 8,396 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,949,129 | 31,949,151 | GTGGGAGACTTAGAGTTGGGTTC |
| TUP | 31,949,155 | 31,949,177 | TTTGTGGCTGTCTGACGGGCATC |
| TUR | 31,949,182 | 31,949,199 | GGCGACTCGTGTGACTTG |
| TDF | 31,956,466 | 31,956,489 | GGGATTCCAGTTACAATACAGGTC |
| TDP | 31,956,491 | 31,956,512 | TTTCGTCAGTTTGTGGTTATTC |
| TDR | 31,956,521 | 31,956,544 | TCAACACTAACAAGCACAAGTTCC |
PCR Primers
| Primer | Sequence |
| SU | GTAGCTGTAGCAGCGCGAAG |
| SD | ATCCAGTTGGGAAACACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17804A-H4 | VGB6 | C57BL/6NTac | |
| 17804A-B3 | VGB6 | C57BL/6NTac | |
| 17804A-B5 | VGB6 | C57BL/6NTac | |
| 17804A-B11 | VGB6 | C57BL/6NTac | |
| 17804A-E7 | VGB6 | C57BL/6NTac | |
