| Gene | Pcbd2 |
| Chromosome | 13 |
| Deletion Start | 55,733,064 |
| Deletion End | 55,727,376 |
| Deletion Size | 5,689 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,727,736 | 55,727,757 | ACGTTTAGTTGCGAAGCCATTC |
| TUP | 55,727,758 | 55,727,777 | TGTGGGCTGCGAGCAGTCCT |
| TUR | 55,727,796 | 55,727,815 | CAACACGGTTAGCTGGTCAC |
| TDF | 55,732,529 | 55,732,550 | CAGAGACGGAAGGTGCTAGAAG |
| TDP | 55,732,552 | 55,732,576 | CCATCCACAGAACATCAAGCCAGCA |
| TDR | 55,732,584 | 55,732,602 | AAGCTGCTGGTGGGACTTG |
PCR Primers
| Primer | Sequence |
| SU | GTAAGGGAACAGAGTTGTC |
| SD | GATAGAGTCTTCCATCTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17807A-B5 | VGB6 | C57BL/6NTac | |
| 17807A-E8 | VGB6 | C57BL/6NTac | |
| 17807A-F4 | VGB6 | C57BL/6NTac | |
| 17807A-C6 | VGB6 | C57BL/6NTac | |
