| Gene | Pdxdc1 |
| Chromosome | 16 |
| Deletion Start | 13,902,932 |
| Deletion End | 13,872,459 |
| Deletion Size | 30,474 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,902,205 | 13,902,223 | GGCAACAGCAGCTTGATGG |
| TUP | 13,902,172 | 13,902,201 | TGGCTACCTACTCGGGCATGTTAAATAGAC |
| TUR | 13,902,134 | 13,902,156 | TGTTCTCAATAGCCAGCCCTTAG |
| TDF | 13,872,555 | 13,872,577 | GTCTCCACTGCTCAGTCTCATAG |
| TDP | 13,872,532 | 13,872,554 | TCATAAGTAAACAGCGTGTTTCT |
| TDR | 13,872,510 | 13,872,530 | AAGGGTAAGCCAAGCTGCAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGGACTTGAAAGCCTGG |
| SD | CATGATGATGAAAGGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17808A-F1 | VGB6 | C57BL/6NTac | |
| 17808A-D5 | VGB6 | C57BL/6NTac | |
| 17808A-B11 | VGB6 | C57BL/6NTac | |
| 17808A-D9 | VGB6 | C57BL/6NTac | |
