| Gene | Rad17 |
| Chromosome | 13 |
| Deletion Start | 100,649,716 |
| Deletion End | 100,617,579 |
| Deletion Size | 32,138 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,649,637 | 100,649,659 | GGTCATGTGGTGATTGCCTAAAG |
| TUP | 100,649,593 | 100,649,622 | TTGTTTGTTCTCAGTGCTTTATGCCTGTCA |
| TUR | 100,649,552 | 100,649,576 | TCAGATCACTGATTGTAACAGACAC |
| TDF | 100,618,468 | 100,618,489 | TGGCTGCTTAAACTCAGAGGTC |
| TDP | 100,618,442 | 100,618,466 | AGTGCCTGCCTTTGCCTCCTGAGTG |
| TDR | 100,618,409 | 100,618,428 | AAGCTGGGCATGGTGCTCAC |
PCR Primers
| Primer | Sequence |
| SU | TACCATCATAGATAGATTATCTG |
| SD | TGCAAACTGTAAGTAAGGCACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17812A-A11 | VGB6 | C57BL/6NTac | |
| 17812A-B4 | VGB6 | C57BL/6NTac | |
| 17812A-G2 | VGB6 | C57BL/6NTac | |
| 17812A-D11 | VGB6 | C57BL/6NTac | |
| 17812A-A12 | VGB6 | C57BL/6NTac | |
| 17812A-H9 | VGB6 | C57BL/6NTac | |
