| Gene | Rad9b |
| Chromosome | 5 |
| Deletion Start | 122,354,172 |
| Deletion End | 122,325,822 |
| Deletion Size | 28,351 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,354,108 | 122,354,131 | GGCCATTCTTGCTTTGCTACTTAG |
| TUP | 122,354,070 | 122,354,095 | TCTAACGCCTCGTGCAGGGTTTATCC |
| TUR | 122,354,049 | 122,354,067 | GAGGAGGCCGAAGAAGACG |
| TDF | 122,325,951 | 122,325,972 | GCATGTTCTTTGGAGCTGTATC |
| TDP | 122,325,924 | 122,325,947 | TGAACAGCAAGAGTATGCCGGCCA |
| TDR | 122,325,876 | 122,325,895 | CTCACGTCCTCCTCACTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCTCACCATCCTGTCACCAG |
| SD | CAGGCCATCACATACACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17813A-C8 | VGB6 | C57BL/6NTac | |
| 17813A-F6 | VGB6 | C57BL/6NTac | |
| 17813A-D11 | VGB6 | C57BL/6NTac | |
| 17813A-D2 | VGB6 | C57BL/6NTac | |
| 17813A-B12 | VGB6 | C57BL/6NTac | |
