| Gene | Scrn3 |
| Chromosome | 2 |
| Deletion Start | 73,335,900 |
| Deletion End | 73,315,437 |
| Deletion Size | 20,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,316,342 | 73,316,362 | TGGACTATGGATGCAACCAAC |
| TUP | 73,316,371 | 73,316,395 | AGTGTAGGATGGTTTAAATGTGGGA |
| TUR | 73,316,396 | 73,316,418 | CCCAACCACTACTCCTACCTAAG |
| TDF | 73,335,186 | 73,335,204 | ACTGTCCGCACGCTTCTTC |
| TDP | 73,335,208 | 73,335,232 | CCCAAGTGAGCTTGCAGTTGCCATG |
| TDR | 73,335,235 | 73,335,257 | GTACCAATGAACAGGCAGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGCCTTTTACTGCCATG |
| SD | CACAAGTTTGCTTGCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17817A-A7 | VGB6 | C57BL/6NTac | |
| 17817A-H4 | VGB6 | C57BL/6NTac | |
| 17817A-G9 | VGB6 | C57BL/6NTac | |
| 17817A-F12 | VGB6 | C57BL/6NTac | |
| 17817A-F6 | VGB6 | C57BL/6NTac | |
| 17817A-A12 | VGB6 | C57BL/6NTac | |
