| Gene | Uqcr11 |
| Chromosome | 10 |
| Deletion Start | 80,406,742 |
| Deletion End | 80,404,135 |
| Deletion Size | 2,608 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,406,612 | 80,406,631 | GGCAGAGTATTGCGGGACAC |
| TUP | 80,406,579 | 80,406,603 | TGGAAGCTCCGAGGTTAAGCTGCAA |
| TUR | 80,406,546 | 80,406,565 | GCTTGGAGCACCTCAGACAC |
| TDF | 80,404,341 | 80,404,363 | GCATGGGATATGATGGGAGAAGG |
| TDP | 80,404,310 | 80,404,339 | TCTCAAAGGCTAAGTTACCAGCAGAGTCTA |
| TDR | 80,404,277 | 80,404,298 | TGTGGGAATCCTGGTAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAAAGTCACACACACTCTC |
| SD | TACACAAGTCAGGTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17822A-A4 | VGB6 | C57BL/6NTac | |
| 17822A-F11 | VGB6 | C57BL/6NTac | |
| 17822A-C3 | VGB6 | C57BL/6NTac | |
| 17822A-E4 | VGB6 | C57BL/6NTac | |
| 17822A-B6 | VGB6 | C57BL/6NTac | |
| 17822A-E10 | VGB6 | C57BL/6NTac | |
