| Gene | Zw10 |
| Chromosome | 9 |
| Deletion Start | 49,078,327 |
| Deletion End | 49,055,686 |
| Deletion Size | 22,642 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,056,431 | 49,056,452 | CCGTGTAATAGCCCTCGTTGTC |
| TUP | 49,056,454 | 49,056,482 | TGGATTCAAGCTTTGTAGAACTGGCTGGT |
| TUR | 49,056,489 | 49,056,508 | GGCAGGCGAATTTCTGTGAG |
| TDF | 49,077,925 | 49,077,947 | GATGGTTATGAGCTGCCATGTAG |
| TDP | 49,077,951 | 49,077,977 | CTGGGAGGTGAATTCTGGTCCTCTGAA |
| TDR | 49,077,993 | 49,078,011 | GCTCCGGCTAAGAGCACTG |
PCR Primers
| Primer | Sequence |
| SU | TCTAGCGAAACCAACCCAAC |
| SD | GTAATGTGCCCTTAATGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17824A-A2 | VGB6 | C57BL/6NTac | |
| 17824A-G9 | VGB6 | C57BL/6NTac | |
| 17824A-C4 | VGB6 | C57BL/6NTac | |
| 17824A-D4 | VGB6 | C57BL/6NTac | |
| 17824A-A5 | VGB6 | C57BL/6NTac | |
