| Gene | 1110002N22Rik |
| Chromosome | 11 |
| Deletion Start | 80,142,116 |
| Deletion End | 80,136,804 |
| Deletion Size | 5,313 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,142,049 | 80,142,068 | CCTGAGCACGAGGTGGATTG |
| TUP | 80,142,021 | 80,142,044 | CCAGTCACTAGCCTGTAACCGGGA |
| TUR | 80,141,996 | 80,142,014 | CGGACACAGGGCAGAAGAG |
| TDF | 80,137,473 | 80,137,495 | GCATGGCTATAAGAGGCTTTGAC |
| TDP | 80,137,447 | 80,137,471 | AGACCCTTGCAACCTGCAGAGTCCA |
| TDR | 80,137,370 | 80,137,390 | CTGACTTCGAGACTCAGCAAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCTCTCAACTCCGGACAG |
| SD | GGCTCCTGTAGGTGTTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17825A-B10 | VGB6 | C57BL/6NTac | |
| 17825A-H11 | VGB6 | C57BL/6NTac | |
| 17825A-C2 | VGB6 | C57BL/6NTac | |
| 17825A-G5 | VGB6 | C57BL/6NTac | |
| 17825A-D6 | VGB6 | C57BL/6NTac | |
