| Gene | 1700021C14Rik |
| Chromosome | 3 |
| Deletion Start | 88,295,424 |
| Deletion End | 88,282,764 |
| Deletion Size | 12,661 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,294,791 | 88,294,812 | GTCCTCAAACTCGAAACAGTCC |
| TUP | 88,294,769 | 88,294,790 | TGCTACAGCCTTCCCAGCTGCT |
| TUR | 88,294,746 | 88,294,768 | AGGTAGTACATGCCTGTCAACTC |
| TDF | 88,283,271 | 88,283,292 | TCTGTGACTTCCTGCTGGATAC |
| TDP | 88,283,236 | 88,283,264 | TCAAGCAGGGAACTTACACAGACATGAGC |
| TDR | 88,283,187 | 88,283,207 | TGCTCTGCTTCTCCTGTAGTG |
PCR Primers
| Primer | Sequence |
| SU | AAAACCAGACACCTCTATGAGA |
| SD | GCAGCTGATGTCAGTGTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17826A-A10 | VGB6 | C57BL/6NTac | |
| 17826A-B5 | VGB6 | C57BL/6NTac | |
| 17826A-B11 | VGB6 | C57BL/6NTac | |
| 17826A-D5 | VGB6 | C57BL/6NTac | |
| 17826A-F9 | VGB6 | C57BL/6NTac | |
| 17826A-D10 | VGB6 | C57BL/6NTac | |
