| Gene | Ccdc57 |
| Chromosome | 11 |
| Deletion Start | 120,921,923 |
| Deletion End | 120,919,478 |
| Deletion Size | 2,446 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,921,534 | 120,921,554 | CAGGAGCATCGCTTGGAGTTG |
| TUP | 120,921,511 | 120,921,532 | AGCGCGTCCACAGGTGCGTTAC |
| TUR | 120,921,481 | 120,921,502 | ACTGGGCACCAGACACAATGAC |
| TDF | 120,919,584 | 120,919,605 | CAACACCCTGTCTGCTTACCAC |
| TDP | 120,919,558 | 120,919,583 | AGTGACAAGAACAGCGAACTGGCCCA |
| TDR | 120,919,528 | 120,919,545 | CACTCCAGGCGCTCATTC |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGCACTTGTATAGTGTA |
| SD | TCCAAGACCTCCAAGGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17827A-A6 | VGB6 | C57BL/6NTac | |
| 17827A-F5 | VGB6 | C57BL/6NTac | |
| 17827A-F9 | VGB6 | C57BL/6NTac | |
| 17827A-F12 | VGB6 | C57BL/6NTac | |
| 17827A-G12 | VGB6 | C57BL/6NTac | |
| 17827A-G2 | VGB6 | C57BL/6NTac | |
