| Gene | Cidec |
| Chromosome | 6 |
| Deletion Start | 113,434,484 |
| Deletion End | 113,425,561 |
| Deletion Size | 8,924 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,433,692 | 113,433,711 | CTGGCAAGCTGAGCATTCTG |
| TUP | 113,433,667 | 113,433,691 | TCACCTGTCGCCTAGCAACTCTGTA |
| TUR | 113,433,638 | 113,433,661 | CTGGGTTCAAGTTCAAAGTTTGTC |
| TDF | 113,425,728 | 113,425,746 | TGAACGCCTTGCTATCTCC |
| TDP | 113,425,699 | 113,425,722 | AGATGCTCCGCTGGACCCTCTTCA |
| TDR | 113,425,667 | 113,425,684 | GCCAAGCAGCATGTGACC |
PCR Primers
| Primer | Sequence |
| SU | CTTGGGTCGGAGAAACAATG |
| SD | TTCAGGCCAAGGTATGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17828A-A11 | VGB6 | C57BL/6NTac | |
| 17828A-C3 | VGB6 | C57BL/6NTac | |
| 17828A-H3 | VGB6 | C57BL/6NTac | |
| 17828A-H2 | VGB6 | C57BL/6NTac | |
| 17828A-E11 | VGB6 | C57BL/6NTac | |
