| Gene | Cnst |
| Chromosome | 1 |
| Deletion Start | 179,606,528 |
| Deletion End | 179,579,393 |
| Deletion Size | 27,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 179,580,018 | 179,580,038 | CAGCCTCCTGTAGGATCTTCC |
| TUP | 179,580,039 | 179,580,059 | TGCCTCCCTCTGCAAGTGAGC |
| TUR | 179,580,067 | 179,580,088 | TCCTCAACACTGGTGCATGTAG |
| TDF | 179,606,059 | 179,606,080 | TGGTGAAGGAAGCGAACACAAG |
| TDP | 179,606,082 | 179,606,105 | TGCAGCTCTCCTTTGACCACGGAG |
| TDR | 179,606,116 | 179,606,137 | AACAGTCCCAGGGCTGAAATAC |
PCR Primers
| Primer | Sequence |
| SU | GAGCACCTAGGAAGGCAGTG |
| SD | AAGCTATTTCAGACCACTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17830A-A8 | VGB6 | C57BL/6NTac | |
| 17830A-H9 | VGB6 | C57BL/6NTac | |
| 17830A-C6 | VGB6 | C57BL/6NTac | |
| 17830A-H3 | VGB6 | C57BL/6NTac | |
