| Gene | Prkcd |
| Chromosome | 14 |
| Deletion Start | 30,610,299 |
| Deletion End | 30,595,847 |
| Deletion Size | 14,453 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,609,590 | 30,609,609 | GCCATGTTCCTTGCCATCAG |
| TUP | 30,609,566 | 30,609,588 | TCATGGCTAACCTGTGGCAGCTC |
| TUR | 30,609,506 | 30,609,526 | CTGCTCCAGCTCCAATACAAC |
| TDF | 30,596,064 | 30,596,085 | GACCTTGGAGACACCCTTTCAG |
| TDP | 30,596,038 | 30,596,062 | CAAGTCACTCTTACCTGCCACTGCC |
| TDR | 30,595,993 | 30,596,013 | TGGGTCAAAGTTGCTGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTGCCTGGGTGTGTATCAG |
| SD | CATCACCAGTCTCCTACATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17836A-B8 | VGB6 | C57BL/6NTac | |
| 17836A-B10 | VGB6 | C57BL/6NTac | |
| 17836A-E5 | VGB6 | C57BL/6NTac | |
| 17836A-F12 | VGB6 | C57BL/6NTac | |
| 17836A-G12 | VGB6 | C57BL/6NTac | |
| 17836A-H2 | VGB6 | C57BL/6NTac | |
