Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17838 Srsf11 Status: ES cell colonies screened / QC positives

Gene	Srsf11
Chromosome	3
Deletion Start	158,023,104
Deletion End	158,011,914
Deletion Size	11,191
Genome Build	38

MGI ID	MGI:1916457
NCBI Gene ID	69207
BAC ID	357K7
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	158,022,958	158,022,980	GTGTTCTCATGCAAGGATGTTTC
TUP	158,022,926	158,022,955	TGCAGGAGTTATTCCTGATGAGACTAAGGC
TUR	158,022,899	158,022,920	GCATTAGCTGGTGCCAATAGAG
TDF	158,012,016	158,012,039	CCACGAAGAAGACATGGATATGAG
TDP	158,011,995	158,012,015	TGACTGAGCCCAAGGCTGGCT
TDR	158,011,971	158,011,993	GGAATGACACTGAGGACCATCTG

PCR Primers

Primer	Sequence
SU	TGCTTTATAGTGAAGCAGCCG
SD	TTGTCATGTCAACAAACAAGACC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	82 bp	+	+	-
TDF	TDR	69 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17838A-B2	VGB6	C57BL/6NTac
17838A-B12	VGB6	C57BL/6NTac
17838A-D10	VGB6	C57BL/6NTac
17838A-D2	VGB6	C57BL/6NTac