| Gene | Taf7 |
| Chromosome | 18 |
| Deletion Start | 37,643,509 |
| Deletion End | 37,642,487 |
| Deletion Size | 1,023 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,643,392 | 37,643,412 | CTGGGCATGTCAACCTGAAAG |
| TUP | 37,643,359 | 37,643,386 | CTGAGCATTGAGTTACACCCTGATGGGC |
| TUR | 37,643,327 | 37,643,345 | GGGCACTCGATCCACTCTG |
| TDF | 37,642,648 | 37,642,668 | GCAAGCTCCAAGAAACCCAAG |
| TDP | 37,642,624 | 37,642,646 | CAGGGCAAAGCGCCAGGAAGATC |
| TDR | 37,642,569 | 37,642,587 | TCCAGAACTGCCTGAAACC |
PCR Primers
| Primer | Sequence |
| SU | CGAAGAGTTCGTTCACTCCC |
| SD | CAAGTGTTTTCATTTCACTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17840A-B7 | VGB6 | C57BL/6NTac | |
| 17840A-F10 | VGB6 | C57BL/6NTac | |
| 17840A-G8 | VGB6 | C57BL/6NTac | |
| 17840A-C12 | VGB6 | C57BL/6NTac | |
| 17840A-E3 | VGB6 | C57BL/6NTac | |
