Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17841 Tkt Status: ES cell colonies screened / QC positives

Gene	Tkt
Chromosome	14
Deletion Start	30,573,524
Deletion End	30,549,277
Deletion Size	24,248
Genome Build	38

MGI ID	MGI:105992
NCBI Gene ID	21881
BAC ID	374K4
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 14

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	30,550,030	30,550,048	CGAGGCCCACGCGAAATAG
TUP	30,550,050	30,550,076	AAGTTCTGGGCTAACGGTGTTTGCACA
TUR	30,550,097	30,550,117	CAGGCCCAATGGAGACCTTAG
TDF	30,572,813	30,572,833	AACTCAAGGAGCAGGGAAGAC
TDP	30,572,834	30,572,859	CCGGCAGTCACATAGCCTTCGAAAGC
TDR	30,572,875	30,572,893	AGAGGCCAGGAGAGGACAC

PCR Primers

Primer	Sequence
SU	GTGTGTGATTAATTCCAACAGG
SD	GTACACTGGCAAAGGAAGGG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	88 bp	+	+	-
TDF	TDR	81 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17841A-A1	VGB6	C57BL/6NTac
17841A-D11	VGB6	C57BL/6NTac
17841A-E3	VGB6	C57BL/6NTac
17841A-F10	VGB6	C57BL/6NTac