| Gene | Utp23 |
| Chromosome | 15 |
| Deletion Start | 51,882,546 |
| Deletion End | 51,877,526 |
| Deletion Size | 5,021 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,877,735 | 51,877,753 | GGTGTGGCCCAGAGTTCTC |
| TUP | 51,877,756 | 51,877,778 | CGGTGCCAACCAAGTCGGTTACC |
| TUR | 51,877,809 | 51,877,828 | TCACAAGGCACAGTACCATC |
| TDF | 51,881,558 | 51,881,580 | TCTCTGCCTTCTGGACTCATAAC |
| TDP | 51,881,583 | 51,881,612 | AGCATGTTCCTGAATTCCTGCTTCTCAGCT |
| TDR | 51,881,630 | 51,881,651 | GCAGCCTCCTGATGCTAATCTC |
PCR Primers
| Primer | Sequence |
| SU | ACAAAGTGCCTGGGTCAATG |
| SD | CCCAACAAGCTCACAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17842A-A3 | VGB6 | C57BL/6NTac | |
| 17842A-A11 | VGB6 | C57BL/6NTac | |
| 17842A-G12 | VGB6 | C57BL/6NTac | |
| 17842A-G4 | VGB6 | C57BL/6NTac | |
| 17842A-E8 | VGB6 | C57BL/6NTac | |
