| Gene | Whsc2 |
| Chromosome | 5 |
| Deletion Start | 33,936,232 |
| Deletion End | 33,898,633 |
| Deletion Size | 37,600 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,935,778 | 33,935,799 | GCTCCGAAGCTACTCTTGGTTC |
| TUP | 33,935,743 | 33,935,772 | TGTTAGTGCAACTTTCCATGTGACTGCTGA |
| TUR | 33,935,698 | 33,935,721 | GCAAGCCGACTGATATTTGGTAAG |
| TDF | 33,899,079 | 33,899,097 | TGCTGCCCAGGAGATGTTC |
| TDP | 33,899,048 | 33,899,071 | CAAACAAGGTCACTCGGCCGGAGA |
| TDR | 33,899,013 | 33,899,031 | TCGAGAGCCAGCCATGAAG |
PCR Primers
| Primer | Sequence |
| SU | ATCATGCGTTACGGAAACCC |
| SD | CCCAGACACAGGTTTGAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17843A-B8 | VGB6 | C57BL/6NTac | |
| 17843A-H12 | VGB6 | C57BL/6NTac | |
| 17843A-E12 | VGB6 | C57BL/6NTac | |
