| Gene | Xpc |
| Chromosome | 6 |
| Deletion Start | 91,515,796 |
| Deletion End | 91,490,052 |
| Deletion Size | 25,745 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,515,502 | 91,515,522 | TGAAGGCTGAGCAGCAGAAAG |
| TUP | 91,515,475 | 91,515,499 | TCGCAGTTACCAGGTGTCTCAAGGA |
| TUR | 91,515,432 | 91,515,450 | TCTCGCACGCACTAGAGTC |
| TDF | 91,490,477 | 91,490,495 | TGCCTGGGCATCTTCTTTC |
| TDP | 91,490,449 | 91,490,475 | ACCAGCAGTTCTCACTTACGTACCTGC |
| TDR | 91,490,410 | 91,490,428 | CTGCCTTGCTGGCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGAAACTCTGGGCCTACG |
| SD | CGTTGTGAACGGCACTAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17844A-A9 | VGB6 | C57BL/6NTac | |
| 17844A-D8 | VGB6 | C57BL/6NTac | |
| 17844A-D10 | VGB6 | C57BL/6NTac | |
| 17844A-G9 | VGB6 | C57BL/6NTac | |
| 17844A-G2 | VGB6 | C57BL/6NTac | |
