| Gene | Coq3 |
| Chromosome | 4 |
| Deletion Start | 21,910,698 |
| Deletion End | 21,879,691 |
| Deletion Size | 31,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,880,124 | 21,880,143 | AGCCGCCATTCAACTTGCTG |
| TUP | 21,880,146 | 21,880,170 | TCCTGGAGGTGGTTCTTGCTTTCCA |
| TUR | 21,880,182 | 21,880,201 | ACGTTCCCCGTGTGTCTTTC |
| TDF | 21,910,428 | 21,910,450 | CAGTTGAAACTGTGGCAGGATTG |
| TDP | 21,910,460 | 21,910,488 | CCTTTCTCAGGTTACTGGCATTGGAGTGA |
| TDR | 21,910,504 | 21,910,522 | CACAGCATGGGCTGCATAG |
PCR Primers
| Primer | Sequence |
| SU | AGCGCATTGAAAGAGAATGG |
| SD | AGTCAAGGGCATTGTATCTTTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17849A-C9 | VGB6 | C57BL/6NTac | |
| 17849A-G4 | VGB6 | C57BL/6NTac | |
