| Gene | Dcaf5 |
| Chromosome | 12 |
| Deletion Start | 80,436,395 |
| Deletion End | 80,404,558 |
| Deletion Size | 31,838 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,435,730 | 80,435,748 | AGACCAAGGCTCGCCAAAG |
| TUP | 80,435,696 | 80,435,718 | CTCGAGCCCCAGTCCTCGGTGTC |
| TUR | 80,435,602 | 80,435,619 | TCTGCTACCCGTCACCAC |
| TDF | 80,404,985 | 80,405,005 | GAACTTGAAGAGGGCTGAGAC |
| TDP | 80,404,954 | 80,404,982 | AACACAGTGGTAGATAGAGCACTTGCCTA |
| TDR | 80,404,935 | 80,404,953 | CCCAGAGCCTTGTGCATAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGGGGATCAGCTGCTG |
| SD | GAAGCATGCACTGTATGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17850A-H5 | VGB6 | C57BL/6NTac | |
