| Gene | Dtx1 |
| Chromosome | 5 |
| Deletion Start | 120,710,310 |
| Deletion End | 120,681,177 |
| Deletion Size | 29,134 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,710,091 | 120,710,111 | AGCTGGTGCCCTACATCATCG |
| TUP | 120,710,062 | 120,710,086 | ACAATCCATGCATCAGTTCCGGCAA |
| TUR | 120,710,044 | 120,710,061 | AGGCCACTCACCTGTGTC |
| TDF | 120,681,431 | 120,681,450 | CCTGGGAACGCAGACTCATC |
| TDP | 120,681,403 | 120,681,429 | TCACTATCGGAACATCCAACACCACGG |
| TDR | 120,681,352 | 120,681,376 | CAAACTCCGTCTTGTGGTGAATCTC |
PCR Primers
| Primer | Sequence |
| SU | CACCTAGGACAGACATTGC |
| SD | ACTCAAGTCCTGGGAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17851A-A2 | VGB6 | C57BL/6NTac | |
| 17851A-C2 | VGB6 | C57BL/6NTac | |
| 17851A-B5 | VGB6 | C57BL/6NTac | |
| 17851A-A11 | VGB6 | C57BL/6NTac | |
| 17851A-A5 | VGB6 | C57BL/6NTac | |
