| Gene | Fancd2 |
| Chromosome | 6 |
| Deletion Start | 113,565,269 |
| Deletion End | 113,533,811 |
| Deletion Size | 31,459 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,534,150 | 113,534,169 | CACATGGCACCCACAATCAC |
| TUP | 113,534,171 | 113,534,200 | TGTAATTTCAGTTCCAGGGATTCAGTGTCC |
| TUR | 113,534,239 | 113,534,257 | GCCTGCCGGAACCTAAATG |
| TDF | 113,564,280 | 113,564,302 | TTCTCCCTTCCCAGATCAATGTC |
| TDP | 113,564,304 | 113,564,328 | TCTTTGTGCCTGGCTTCCCATTTCC |
| TDR | 113,564,334 | 113,564,354 | TCTTGCCACGCAAAGTCTCAG |
PCR Primers
| Primer | Sequence |
| SU | GACGAGTTATGGGCTTTCTCC |
| SD | CAGGGGCCTGTAGCAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17852A-A6 | VGB6 | C57BL/6NTac | |
| 17852A-D3 | VGB6 | C57BL/6NTac | |
| 17852A-F12 | VGB6 | C57BL/6NTac | |
| 17852A-G1 | VGB6 | C57BL/6NTac | |
| 17852A-G2 | VGB6 | C57BL/6NTac | |
| 17852A-H7 | VGB6 | C57BL/6NTac | |
