| Gene | Lpcat3 |
| Chromosome | 6 |
| Deletion Start | 124,698,175 |
| Deletion End | 124,663,212 |
| Deletion Size | 34,964 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,663,647 | 124,663,667 | TGCGACCTATGTCTGTCTGTG |
| TUP | 124,663,670 | 124,663,695 | TCTCAAGAGTTCGACTGTGGCCATGA |
| TUR | 124,663,738 | 124,663,758 | AGGGCCTATGCCTCTCGTAAG |
| TDF | 124,698,026 | 124,698,045 | GTCAGCAGGCATGAGTAACC |
| TDP | 124,698,052 | 124,698,077 | TCTTCTCCTCCCATCTAGGCTACCCG |
| TDR | 124,698,129 | 124,698,150 | CCGTGAAGGTGTGGAAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | TGCTCCTGTCTCAACCAATG |
| SD | TTACTGTTTTTCCACATCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17853A-A7 | VGB6 | C57BL/6NTac | |
| 17853A-H6 | VGB6 | C57BL/6NTac | |
| 17853A-F8 | VGB6 | C57BL/6NTac | |
