| Gene | Lrriq4 |
| Chromosome | 3 |
| Deletion Start | 30,672,381 |
| Deletion End | 30,645,333 |
| Deletion Size | 27,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,645,491 | 30,645,510 | ACCAGAGACAGTGGGCAGAC |
| TUP | 30,645,511 | 30,645,537 | TGTGACTCCAACCAGCATCCTATCTGA |
| TUR | 30,645,553 | 30,645,575 | ACCAAGCCCAACACATGATAGAG |
| TDF | 30,672,016 | 30,672,040 | CTCATGCTTGCACAGAAAATACTAC |
| TDP | 30,672,043 | 30,672,066 | TCTACTGCATGCCCCAACCCTTCA |
| TDR | 30,672,080 | 30,672,101 | ATGGTCTAGCTGTGGCTTACTC |
PCR Primers
| Primer | Sequence |
| SU | CCCATGTCAGATACCACTCC |
| SD | TGGTGATTTATGAAGAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17854A-A3 | VGB6 | C57BL/6NTac | |
| 17854A-A6 | VGB6 | C57BL/6NTac | |
| 17854A-B11 | VGB6 | C57BL/6NTac | |
| 17854A-C10 | VGB6 | C57BL/6NTac | |
| 17854A-D9 | VGB6 | C57BL/6NTac | |
| 17854A-B5 | VGB6 | C57BL/6NTac | |
