| Gene | Pecr |
| Chromosome | 1 |
| Deletion Start | 72,284,213 |
| Deletion End | 72,259,336 |
| Deletion Size | 24,878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,284,019 | 72,284,041 | GGGTAAACTGGGTTCAGAGACAG |
| TUP | 72,283,979 | 72,284,002 | ACCGGGAAGAGGTTGCAAAGCTCC |
| TUR | 72,283,895 | 72,283,916 | GATCAGGGCCTCTCAACATTTC |
| TDF | 72,259,930 | 72,259,951 | GTCATCACCATTGGCCTTTCAG |
| TDP | 72,259,902 | 72,259,929 | TCTCCATGAAGAGGTGGGACACATCTAA |
| TDR | 72,259,863 | 72,259,885 | ACTTCACCAGAACAAACACAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCATTCCTCTGTCGTTTCCC |
| SD | CCTTCCTCCAACATACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17855A-F6 | VGB6 | C57BL/6NTac | |
| 17855A-B8 | VGB6 | C57BL/6NTac | |
| 17855A-F1 | VGB6 | C57BL/6NTac | |
| 17855A-F11 | VGB6 | C57BL/6NTac | |
| 17855A-H4 | VGB6 | C57BL/6NTac | |
