| Gene | Taco1 |
| Chromosome | 11 |
| Deletion Start | 106,073,351 |
| Deletion End | 106,066,251 |
| Deletion Size | 7,101 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,066,405 | 106,066,424 | CCTCGGCGGGACACAATAAG |
| TUP | 106,066,425 | 106,066,450 | TGGTCTAAAGTCCGGCACATCAAGGG |
| TUR | 106,066,488 | 106,066,508 | CAGGCGGATACTGAGAGTGAG |
| TDF | 106,073,160 | 106,073,181 | GTGTCCTGTGTCCTGTTCAATG |
| TDP | 106,073,187 | 106,073,209 | CATCCCTCACTCCAAGGTGCAGC |
| TDR | 106,073,242 | 106,073,263 | AGTTGTTAAGCGCCTGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGTCGTTTCCAAGCAACC |
| SD | CATGTGGGCTTGAATGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17859A-G6 | VGB6 | C57BL/6NTac | |
| 17859A-B9 | VGB6 | C57BL/6NTac | |
| 17859A-C12 | VGB6 | C57BL/6NTac | |
| 17859A-F7 | VGB6 | C57BL/6NTac | |
| 17859A-E9 | VGB6 | C57BL/6NTac | |
| 17859A-A1 | VGB6 | C57BL/6NTac | |
