| Gene | Tsx |
| Chromosome | X |
| Deletion Start | 103,424,302 |
| Deletion End | 103,414,556 |
| Deletion Size | 9,747 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,414,841 | 103,414,864 | TCTCCAAAGCTGTCAAACTAAACC |
| TUP | 103,414,871 | 103,414,898 | TCCCTTTAACTGGAGGCAACCAGAATGA |
| TUR | 103,414,899 | 103,414,917 | TCATTCGCAACCGTCTGTG |
| TDF | 103,423,878 | 103,423,898 | GCACCCTCACAGGTTTGACAG |
| TDP | 103,423,901 | 103,423,927 | TGCAACTTAACCAGCTGCATCTCCAGC |
| TDR | 103,423,930 | 103,423,951 | GCCTACGTGAAAAGGCAGTATG |
PCR Primers
| Primer | Sequence |
| SU | ACAAGCTCCATGGTTGGAAG |
| SD | AATTAGCATGACAAGGAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17860A-A7 | VGB6 | C57BL/6NTac | |
| 17860A-B5 | VGB6 | C57BL/6NTac | |
| 17860A-B8 | VGB6 | C57BL/6NTac | |
| 17860A-D10 | VGB6 | C57BL/6NTac | |
