| Gene | Txndc11 |
| Chromosome | 16 |
| Deletion Start | 11,134,506 |
| Deletion End | 11,104,568 |
| Deletion Size | 29,939 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,133,701 | 11,133,724 | GCAGGGAATAGAAGGATGAGGTTG |
| TUP | 11,133,673 | 11,133,697 | AGGGCAGAAAGGGCCTTCATAGGAA |
| TUR | 11,133,646 | 11,133,668 | TTCCTTGTGTAAGTGGCTAATGC |
| TDF | 11,105,405 | 11,105,426 | TGCTAGGGTCTTTAGGCAGATG |
| TDP | 11,105,376 | 11,105,404 | TGATTGTCACAGAACCAAACCAGACAGGG |
| TDR | 11,105,342 | 11,105,363 | TGCTTTGCTTGCTGTCTTTGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTCGCTACAAGAGAGGCG |
| SD | GCTACATCTAATAAACAATAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17861A-F9 | VGB6 | C57BL/6NTac | |
| 17861A-G10 | VGB6 | C57BL/6NTac | |
| 17861A-G6 | VGB6 | C57BL/6NTac | |
| 17861A-G9 | VGB6 | C57BL/6NTac | |
| 17861A-C12 | VGB6 | C57BL/6NTac | |
| 17861A-A9 | VGB6 | C57BL/6NTac | |
