17863 Anp32b Status: Cancelled
| Gene | Anp32b |
| Chromosome | 4 |
| Deletion Start | 46,472,191 |
| Deletion End | 46,451,299 |
| Deletion Size | 20,893 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,451,510 | 46,451,530 | GCGCGGGCTTTAACTCTTTGG |
| TUP | | | CCCGCGGCCACGGGGAG |
| TUR | 46,451,571 | 46,451,591 | GCGGCTCAAAGTTTGCTTCTC |
| TDF | 46,471,533 | 46,471,550 | TGTGTTGCTGGGCTTGTC |
| TDP | 46,471,551 | 46,471,579 | TGAAATTCAGTGTTCCTGCCTTCCCTTCC |
| TDR | 46,471,590 | 46,471,614 | CCAAAGTCTAACAGAGCCATGAATC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGCTTAAGGCGGGTCC |
| SD | GCAGTTATCACACAAAATGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
