17864   Brd3     Status: ES cell colonies screened / QC positives

GeneBrd3
Chromosome2
Deletion Start27,464,095
Deletion End27,448,455
Deletion Size15,641
Genome Build38
MGI IDMGI:1914632
NCBI Gene ID67382
BAC ID416L6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF27,463,88427,463,901GCTGAACCTGCCTGTGAG
TUP27,463,85527,463,883TGGCCCTTAGTGTCTGAGATATAGCAGGG
TUR27,463,81427,463,832AACCACCTTGCCAGACCAC
TDF27,448,57327,448,593TGTGTCCTTGCAGAGAAGTCC
TDP27,448,54827,448,569TCAGCTCCCTCAGGAGGCCCAT
TDR27,448,52727,448,547AGCTGCTACTGCTGAGCCTTG

PCR Primers

PrimerSequence
SUTTTCTGGGAGCTGGTGACTC
SDTTCCAGAGAAACACCCTTGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR88 bp++-
TDFTDR67 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17864A-A9VGB6C57BL/6NTac
17864A-B5VGB6C57BL/6NTac
17864A-E5VGB6C57BL/6NTac
17864A-E10VGB6C57BL/6NTac
17864A-G8VGB6C57BL/6NTac
17864A-H7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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