| Gene | Fabp5 |
| Chromosome | 3 |
| Deletion Start | 10,016,182 |
| Deletion End | 10,012,643 |
| Deletion Size | 3,540 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,013,317 | 10,013,335 | GGCGAAGTGCGGAGACATC |
| TUP | 10,013,339 | 10,013,363 | TGTGCATGGTTGTTGTCTTGCGACC |
| TUR | 10,013,387 | 10,013,406 | GCTCCTCACCCTCCAGAATC |
| TDF | 10,015,591 | 10,015,608 | GGCACCATGCTGGGATTG |
| TDP | 10,015,611 | 10,015,637 | CCTGCAGCCACAGTTGTCATAACCATC |
| TDR | 10,015,661 | 10,015,684 | ATTGTCCTCCTAAGTTTCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CAATTCCTATAGCGCCAAGC |
| SD | AAGGCAAGCTTAAATGACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17865A-C11 | VGB6 | C57BL/6NTac | |
| 17865A-D2 | VGB6 | C57BL/6NTac | |
| 17865A-E3 | VGB6 | C57BL/6NTac | |
| 17865A-E4 | VGB6 | C57BL/6NTac | |
| 17865A-F9 | VGB6 | C57BL/6NTac | |
| 17865A-G8 | VGB6 | C57BL/6NTac | |
