| Gene | Fkbp1a |
| Chromosome | 2 |
| Deletion Start | 151,559,667 |
| Deletion End | 151,542,711 |
| Deletion Size | 16,957 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,543,306 | 151,543,325 | GAAACGACCCACTTGCGAAG |
| TUP | 151,543,326 | 151,543,351 | TCATTATTGTGGCTTCACGTGCCCTC |
| TUR | 151,543,417 | 151,543,439 | ACATCCTTTGGTCTGGAAATCAC |
| TDF | 151,558,715 | 151,558,733 | ATGCCCTGGATCACCACAC |
| TDP | 151,558,735 | 151,558,758 | CAGGCCTTGGGTGTTCACATTGCA |
| TDR | 151,558,762 | 151,558,785 | GTTGCTTTCAGAGACATAACAGAC |
PCR Primers
| Primer | Sequence |
| SU | GGTCTGTGGCCTCCGGAC |
| SD | GAAAGAGCAACTCAGGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17866A-B1 | VGB6 | C57BL/6NTac | |
| 17866A-A4 | VGB6 | C57BL/6NTac | |
| 17866A-G10 | VGB6 | C57BL/6NTac | |
