| Gene | Gm16515 |
| Chromosome | 11 |
| Deletion Start | 60,913,722 |
| Deletion End | 60,905,947 |
| Deletion Size | 7,776 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,912,889 | 60,912,911 | TCGGCCAGTAGGTAGGTTGTAAG |
| TUP | 60,912,861 | 60,912,888 | CAGTCAAGCATACGCAGCCTATCCTGAC |
| TUR | 60,912,826 | 60,912,847 | CTCCAAGTGCAGGTCACAGAAG |
| TDF | 60,906,637 | 60,906,656 | CGCCTTGGGACTAGGACTTG |
| TDP | 60,906,601 | 60,906,628 | TACTTGCTTAGTTAGCCCTGGTGATGCA |
| TDR | 60,906,572 | 60,906,592 | AGGCCACTGACTTGGACACTC |
PCR Primers
| Primer | Sequence |
| SU | GCATATGGGTCCGTGTGG |
| SD | AGCACTGGTTGTGGCTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17867A-D3 | VGB6 | C57BL/6NTac | |
| 17867A-E12 | VGB6 | C57BL/6NTac | |
| 17867A-F9 | VGB6 | C57BL/6NTac | |
| 17867A-A12 | VGB6 | C57BL/6NTac | |
