| Gene | Map1lc3b |
| Chromosome | 8 |
| Deletion Start | 121,596,862 |
| Deletion End | 121,590,528 |
| Deletion Size | 6,335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,591,300 | 121,591,318 | CCTTTGAGGAGGCCAGGAC |
| TUP | 121,591,319 | 121,591,346 | CGGTCTTGCTCCTTTCCAGTGAGTTTGG |
| TUR | 121,591,371 | 121,591,391 | CGCAGTCTGGATAGTGGAAAC |
| TDF | 121,596,165 | 121,596,185 | CAGGTCCTTCCTGCTGCTTTC |
| TDP | 121,596,189 | 121,596,211 | CCCTGTAAGTCAGTGCCCGGCCT |
| TDR | 121,596,252 | 121,596,272 | GACAACCCTTTGGTCCCTCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGATGCTCGCCCAGCGTG |
| SD | AACACCAGACCTGCTTAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17868A-A1 | VGB6 | C57BL/6NTac | |
| 17868A-H4 | VGB6 | C57BL/6NTac | |
| 17868A-E8 | VGB6 | C57BL/6NTac | |
| 17868A-D9 | VGB6 | C57BL/6NTac | |
| 17868A-B4 | VGB6 | C57BL/6NTac | |
| 17868A-G3 | VGB6 | C57BL/6NTac | |
