| Gene | Nab2 |
| Chromosome | 10 |
| Deletion Start | 127,666,455 |
| Deletion End | 127,661,654 |
| Deletion Size | 4,802 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,665,629 | 127,665,648 | GGGCAGCCTTTGGAAAGTTG |
| TUP | 127,665,591 | 127,665,620 | TCTAGGAAGGGTGCTTTAATCCTTGGAAGC |
| TUR | 127,665,570 | 127,665,590 | AGACCCTTGCTCCTGTTCTTC |
| TDF | 127,661,836 | 127,661,855 | GTGCCTGGCCTCATAACAAC |
| TDP | 127,661,811 | 127,661,833 | TTCTGCTCAGCCTCCAACCCAGT |
| TDR | 127,661,768 | 127,661,790 | CCTCCTCGAACTCTGTAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAACTCAGCGCCCGATTC |
| SD | AGTTCAGCTGCTCACACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17869A-C4 | VGB6 | C57BL/6NTac | |
| 17869A-D1 | VGB6 | C57BL/6NTac | |
| 17869A-G4 | VGB6 | C57BL/6NTac | |
| 17869A-F12 | VGB6 | C57BL/6NTac | |
