| Gene | Nckap5l |
| Chromosome | 15 |
| Deletion Start | 99,434,147 |
| Deletion End | 99,422,605 |
| Deletion Size | 11,543 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,433,275 | 99,433,294 | CACTTGGCCCATGCCACTAC |
| TUP | 99,433,245 | 99,433,271 | TGATTGGCCCGTTATTCTCTACCAGGA |
| TUR | 99,433,219 | 99,433,240 | ACCTGGCCTTAACAACTCACTC |
| TDF | 99,422,743 | 99,422,765 | GGAGACCTCAGAATCCCTAAGTG |
| TDP | 99,422,712 | 99,422,739 | CCCTCTATGACTCGCTATCCTCCTGTGG |
| TDR | 99,422,663 | 99,422,682 | CCCTAGCTCCAGAGGAACTG |
PCR Primers
| Primer | Sequence |
| SU | ACGTTGAGTCACAGGGCAG |
| SD | ACCATTCAGACAGCAGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17870A-D7 | VGB6 | C57BL/6NTac | |
| 17870A-E4 | VGB6 | C57BL/6NTac | |
| 17870A-E11 | VGB6 | C57BL/6NTac | |
